Study of the action of quercetin in the liver and brain tissue of white rats on the expression of the superoxide dismutase (SOD1) gene

Author: Mariam Goliadze
Keywords: Quercetin, Gene exspression, SOD1, RT qPCR
Annotation:

Free radicals _ like Reactive Oxygen Species are generated by living organisms as a result of normal metabolism and acting as a signal molecules in the cellular pathway. A disbalance between free radicals and antioxidants can cause “oxidative stress” which contributes to many pathological conditions and diseases. Aerobic organisms have integrated antioxidant systems, which include enzymatic and nonenzymatic antioxidants that are usually effective in blocking harmful effects of ROS. In view of all the above, in order to determine the antioxidant status of a living organism, it is important to study the both action of enzymatic and non-enzymatic antioxidants separately, as well as studing of mechanisms of their interaction. Aim of work: To determine the influence of non-enzymatic antioxidants on the enzymatic antioxidants _study of the action of quercetin in the liver and brain tissue of white rats on the expression of the superoxide dismutase (SOD1) gene. Object of research: White Rats. Methods: RNA extraction, quantitative and qualitative analyse of extracted RNA, reverse transcription, RT qPCR_ Quantitative reverse transcription Polymerase chain reaction. Results: Within the norm range, the expression of the gene encoding superoxide dismutase_1 in both intact and experimental group animals is more intense in the liver than in the brain tissue. Five days injection of quercetin at final concentration of 20mg/kg, did not cause a statistically significant change on the expression of SOD1 gene.

Lecture files:

აბსტრაქტი [ka]