Study of the actiion of flavonoids fraction in rat’s brain and liver on the expression of the genes of some antioxidant enzymes

Author: Nino Gersamia
Keywords: Gene Expression; Superoxide Dismutase; Catalase, Flavonoids, Antioxidant System.
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Reactive Oxigen Species (ROS) produced by aerobic organisms result of normal cellular metabolism and some environmental factors. Over amount of ROS, can damage cell structures, such as nucleic acids, proteins, and lipids and alter their function. Against the ROS, Aerobic organisms have integrated antioxidant systems, which include enzymatic and nonenzymatic antioxidants. There are many researches and studies about their separate functioning, but scarce information is about interdependence of these compounds. The aim of our research was study of interdependence enzymatic and nonenzymatic antioxidants, such as winery flavonoids, including flavonoids, which isolated from Georgian Sapheravi Wine and study of their influence on intensity antioxidant enzymes – superoxide dismutase and catalase genes expression. Because identify of molecules, which have regulatory effects on antioxidant capacity of Aerobic organisms is very crucial for prevention of many pathological processes initiate by antioxidative stress. Several step experiment conducted, of the initial stage, we researched antioxidant properties of flavonoid fraction, isolated from Sapheravi red wine, more precisely, we learned total amount of polyphenols, flavonoids and antioxidant activity of fraction via luminol chemiluminescence method. On the second stage, study continued in vivo, we determined of superoxide dismutase and catalase genes expression intensity in brain and liver tissues of research laboratory rats, without any pathologies or stress. Results showed, that in intact rats, the intensity level of superoxide dismutase 1 (SOD1) and superoxide dismutase 3 (SOD3) genes expression is higher in the liver, compared to the brain, while the degree of expression of the superoxide dismutase 2 (SOD2) gene is similar in both tissues. In rats, a five day injection of quercetin and flavonoid fraction, at final concentration of 20mg/kg, did not cause a statistically significant change on SOD1 and SOD2 genes expression. However, intensity of SOD3 gene expression decreased in liver of research groups, compared to control group. But, relative quantity of SOD3 gene expression is equal in all three groups. It is also noted, that quantification of SOD2 gene expression in brain tissue of research groups is higher than in liver, but in intact rats, this feature is similar in both tissues. The level of catalase gene expression is significantly higher in the rat’s liver compared to the brain in all groups. However the action of quercetin reduces the expression of the catalase gene, in liver tissue.

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აბსტრაქტი [ka]