The Isolation and Comparative Study of Mice Bone Marrow Growth-Inhibiting Thermo-Stable Protein Complex

Author: nino maziashvili
Co-authors: E.Bakuradze. I.Modebadze, L.Rusishvili, N.Bedineishvili
Keywords: white mice, bone marrow, growth factor, mitotic index
Annotation:

The Isolation and Comparative Study of Mice Bone Marrow Growth-Inhibiting Thermo-Stable Protein Complex Anotation The thermostable protein complex (TPC) has been isolated from the bone marrow of adult white mice. The comparative analysis of its components has been performed by polyacrilamide gel electrophoresis. It was established that similarly to the TPCs isolated from other organs, TPC from bone marrow consists of two protein components with the molecular mass of 45-60 kDa and 12-14 kDa. At the same time the low-molecular component ( 12-14 kDa) of TPC is in minor content. It was determined that bone marrow TPC decreases the proliferation activity of homotypic cells. In particular, it inhibits the mitotic activity of homotypic cells by 15%, that could be due t the minor content of low-molecular component. The inhibitory effect of the bone marrow endogenic protein complex is not elicited either in heterotypic, or homotypic cells of growing animals, that points to the fact, that bone marrow TPC of adults retains the tissue-specificity toward the actively proliferating cells of growing organisms. That can be explained with the special functional significance of the bone marrow as the hematopoietic organ in growing, as well as in adult animals.