Molecular Detection of Erythromycin resistant genes in Group B streprococci strains

Annotation GBS- Group B Streptococcus or Streptococcus agalactiae,is a gram-positive coccus which is the harmless inhabitant of the normal microflora. However it was considered as the main reason of neonatal invasions, immediately after its discovery. It is surrounded by the polysaccharide capsule which is the major virulence factor and according to its immunological activity. 10 serotypes of Group B Streptococcus are known. The treatment of infections caused by Group B Streptococcus is mostly carried out by penicillin-G, but erythromycin, vancomycin and clindamycin are recommended for the patients who are allergic to penicillin. In this work I have studied Streptococcus agalactiae strains released from vaginal, urogenital and rectal smears, I have studied sensitivity to antibiotics using the disk diffusion method. Using specific primers I defined of what kind of mechanism. Resistance the given strains demonstated. Streptococcus agalactiae is quite widespread in Georgia. The study of bacterial strains used in the research showed that the number of erythromycin-resistant strains is high. This is turn appears to be a problem for the treatment of patients allergic to penicillin. In such case only vancomycin remains. Fortunately vancomycin resistant strains were not revealed in the course of my research, although the problem is still important. Received data can be used as the basis for the further epidemiological research.