Influence of long circadian rhythm disturbances on the number of some clock genes products

It is known that a disturbance of the natural daily rhythm is a prerequisite for multivariate changes in cell metabolism. The intensity of the cellular energy metabolism and synthetic reactions decrease. In general, the disturbance of the natural daily rhythm causes stress reactions in the body. This includes the constant activation of the hypothalamus-pituitary--that the circadian and stress systems interact. Numerous studies at the Department of Biochemistry have confirmed the stimulating effect of creatine on the energy metabolism of cells and the activity of antioxidative enzymes in the cessation of natural circadian rhythm disturbances. The present study examines the quantitative change in protein products of clock genes in hippocampal cells both in the case of stress due to natural circadian rhythm disturbances and exogenous creatine addition, to establish a connection (if any) between stress and changes in energy metabolism and the clock proteins. The study found that the number of transcription factors synthesized by clock genes under conditions of 30-day circadian rhythm disturbance changed differently, although exogenous creatine input did not affect their quantitative rate. Based on the results obtained, it is hypothesized that quantitative changes in transcription factors are not the reason for the activation of mitochondrial enzymes under creatine addition.