Study of the effect of the endogenous protein complex on GAD-positive and insulin-positive cells of the pancreas of adolescent rats

Author: Magda Lomidze
Keywords: Thermostable protein complex, GAD-positive cells, insulin-positive cells.
Annotation:

Adult rat various tissue (kidney, heart, brain, pancreas, liver) contains a thermostable protein complex (TPC), with the similar properties. It has been shown that this complex accelerates the cells entry into the mitotic cycle and decrease GAD-positive cells in the brain tissue. GAD (glutamate decarboxylase) is an enzyme which is expressed in the central nervous system and in the pancreatic islets, mainly β-cells. It is also a major antigen in the development of autoimmune diabetes. Based on this the goal of our work was to study the effect of pancreatic TPC on the GAD-expression of adolescent rats’ pancreas. It has been revealed that the pancreas TPC causes GAD-positive cells to decrease by approximately 20% in the endocrine part of the pancreas but does not affect the number of insulin-positive cells. The obtained results suggest that inhibition of GAD expression by TPC does not affect the process of insulin production in the same cells. Conclusions. 1. Adult rat pancreatic TPC has ability to inhibit GAD expression in adolescent rat pancreatic β-cells. 2. The endogenous growth factor inhibitory impact does not negative affect on insulin expression.