Production of antibiotic DPPC / DPPA liposomes and their action on the bacterium Escherichia coli (E.coli

Based on the experiments and the results obtained, we found that one of the stage of E.coli bacterial multiplicate, stacionery stage is very interesting of bacterial live activity. In particular, we found that upon reaching the stationary stage of reproduction, several different types of E.coli bacteria are formed that are carriers of different properties. Given the fact that if we take a bacterial culture (so-called night culture) at the beginning of the stationary stage and transfer it to the LB broth, we get that the so-called bacterial culture multiplies. The characteristic curve of the lag phase will be similar to simple S. If we compare the Lag phase of the bacterium, but a few days (more than 2 days) after the inpatient regimen, we get a very difficult, multi-stage initial Lag phase. Interesting results were obtained on the action of antibiotics of different action on E.coli bacteria (Ampiox, Gentamicine, Ceftriacsone) on the bacterium E.coli, the effects of which were compared with those antibiotics, which were placed in the drug DPPC, DPPA liposomes, which were used to treat Efficacy of container action on bacteria. Based on the experiments, it was found that the effectiveness of the antibiotic liposomes on the bacteria was different, but Ceftriacsone's DPPC, DPPA complex with liposomes proved to be more effective than the remaining liposomal antibiotics. The above experimental results were made possible by the use of a new biophysical turbidimeter, which has the ability to observe the interactions of bacteria and antibiotics in a continuous time regime.