Determination of the mechanism of biofilm production of Pseudomonas aeruginosa by biophysical methods

For studying the formation of bacterial biofilms, Zym Croser's modified automatic rotation viscosimetre were used and also a turbidimeter that evaluates the fluid transparency level. The experiment was carried out with different concentrations of bacteria that should be noted with the fact, that this type of research has not been conducted in any of the world's laboratory centers and observation of the biofilm formation process is an unique method of biophysical method. This is proof of that important conclusions have been made on the basis of the established conclusions. The first and most important thing is to develop a completely new biophysical method (viscosimetric), through which we look in real-time the bacterial biofilm formation process, Based on the theoretical analysis of the results obtained from the experiment, two mechanisms are involved in the formation of biofilms , also was found that between the initial concentration of bacteria and the formation of biofilm is a intresting dependence: the bacterium must be in the stationary phase of the reproductive process, so it is necessary to obtain a definite concentration of bacteria. In order to form biofilm, the bacterium in a stationary phase synthesizes specific amphiphilic molecules, that forms centers on air / fluid contact surface from which the biofilm formation process begins. These conclusions are an important step in improving biopharmaceutical processes and provide us with the possibility to plan and conduct more in-depth observations and research on biofilm formation process.