Investigation of creatine’s modulatory effect under the long-term psycho-emotional stress

Author: Temuri Mantashashvili
Keywords: Creatine, psycho-emotional stress, Calcium, Brain, Nitric Oxide, Na+/K+-atp-ase.
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Stress represents a global problem of the modern world, which is responsible for a body reaction to adaptation, behavior, and physiology. The range of assays reveals a tight connection between a continuous change in circadian rhythm and chronic stress. Natural circadian rhythm disruption represents an important risk factor, which can negatively affect a living organism. Similar external factors influence metabolic processes as well as the quantitative change of signaling agents amongst which the Ca2+ takes place. It is known that the quantitative changes of Ca2+ negatively affect cell functioning, particularly, the increase of the Ca2+ gives rise to oxidation processes. According to the complexity of the problem, it is important to obtain the biologically active compounds that would serve as protectors to some extent, such as a nitrogen compound creatine, which is responsible for the transportation and preservation of highly energetic phosphates in a cell. This research aimed to learn the quantitative changes of Ca2+ and NO, observe the Ca-ATP-ase, Mg-ATP-ase, Na/K-ATP-ase, and NO-synthase. It was shown experimentally that the Ca2+ concentration was increased in the stressed rats (G2) compared to the control group (G1), but in the case of injecting creatine, (G3) the concentration approaches the initial (control) amount. It should as well be noted that the circadian rhythm routine disruption increases the number of active compounds, such as nitrogen monoxide and hydrogen peroxide, which were as well positively affected while administering creatine. According to the obtained results, it is shown that the oxidational stress, which is caused by continuous routine disruption, may be initiated as a result of the Ca2+ increase in brain cells. The modulatory effect of creatine positively affects energetic metabolism as well as anti-oxidation activity, which protects hippocampus cells from damaging.

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